Journal: Molecules

Article Title: Pogostemon cablin Triggered ROS-Induced DNA Damage to Arrest Cell Cycle Progression and Induce Apoptosis on Human Hepatocellular Carcinoma In Vitro and In Vivo

doi: 10.3390/molecules25235639

Figure Lengend Snippet: PPa extract stimulated mitochondrial membrane potential loss. ( A ) Mitochondrial membrane potentials (MMPs) were measured at FL2-H and FL1-H channels after PPa extract treatment in HCC cells. ( B ) After PPa extract treatment, JC-1 fluorescence was detected. *: Significant difference between the control group and experimental group; p < 0.05.

Article Snippet: After the cells harvesting, cells stained with JC-1 probe (AAT Bioquest, Inc., Sunnyvale, CA, USA) according to the manufacturer’s instructions.

Techniques: Fluorescence

Journal: PLoS ONE

Article Title: Involvment of Cytosolic and Mitochondrial GSK-3β in Mitochondrial Dysfunction and Neuronal Cell Death of MPTP/MPP + -Treated Neurons

doi: 10.1371/journal.pone.0005491

Figure Lengend Snippet: (A) TSM1 neurons were treated with the indicated concentrations of MPP + for 8 h then cells were stained with JC-1 and analyzed by flow cytometry. (B) TSM1 cells were cultured for 24 h then transfected with scrambled or GSK-3β-specific siRNA (200 nM). Two days post-transfection, cells were pre-treated with saline buffer or 25 mM LiCl or 25 µM kenpaullone ( kenp ), then co-incubated or not with MPP + (400 µM) for 8 h. Cells were stained with JC-1 as described in and prepared for flow cytometry analysis. The results are expressed as the percent of cells with depolarized mitochondrial membrane potential (Ψm). (C, D) intact primary neurons were pre-treated of not with 25 mM LiCl, then co-incubated or not with MPP+ (400 µM) for 4 h. Cells were placed on the stage on a laser-scanning confocal microscope and images were collected every 10 seconds for 10 minutes. One minute after the beginning of experiment, neurons were loaded with 30 nM TMRM (arrow) and one minute before the end of recording, 5 µM FCCP was added to the incubation medium (arrow). (C) Primary neurons pictures were collected 10 minutes after TMRM ( green ) and Hoechst dye ( red ) was added to the incubation medium. Magnification, ×63. (D) The fluorescence intensity plots were obtained by selecting eight roi and expressed as the mean±S.D. of the mean gray values derived from all the selected roi in each image.

Article Snippet: To measure the mitochondrial membrane potential, TSM1 cells were stained with fluorescent probe JC-1 (5,5′, 6, 6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazole carbocyanide iodide, BD Biosciences) at 1.0 µg/mL for 15 min at 37°C, washed and analyzed by flow cytometry.

Techniques: Staining, Flow Cytometry, Cell Culture, Transfection, Incubation, Microscopy, Fluorescence, Derivative Assay